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The embryos(6-8mm) isolated from seeds of Diospyros kaki were cultured on Murashige-Skoog (MS), Woody Plant Medium(WPM), Campbell Durzen(CD), Lictvay´s Medium(LM), Kao-Michaluk (KM), Nitsch, White, Helle;Wolter-Skoog(WS) media. The results showed that MS and WPM media were most suitable to the development of embryos into plantlets with length of 5.4 ± 1.2 cm and 5-6 leaves. Howeve;when LM and KM media were used, the addition of 1 to 2 pmoles/B GA3 was required for the germination of the embryos. Superoxide dismutase(SOD) activities, one of the changing factors in leaves according to physiological status displayed to be exceptionally significant in the leaves of plantlets germinated from seeds in potting sand soil contrary to those of cultured embryos specially around germination period.
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